Hemin-dependent growth and hemin binding of Bartonella henselae
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چکیده
منابع مشابه
Hemin-binding surface protein from Bartonella quintana.
Bartonella quintana, the agent of trench fever and a cause of endocarditis and bacillary angiomatosis in humans, has the highest reported in vitro hemin requirement for any bacterium. We determined that eight membrane-associated proteins from B. quintana bind hemin and that a approximately 25-kDa protein (HbpA) was the dominant hemin-binding protein. Like many outer membrane proteins, HbpA part...
متن کاملHemin binding, functional expression, and complementation analysis of Pap 31 from Bartonella henselae.
Growth of Bartonella henselae is strongly heme dependent, and B. henselae is unable to synthesize heme itself. At least five outer membrane-associated proteins from B. henselae bind hemin, including the 31-kDa protein designated Pap31. The gene of this protein was heterologously expressed in Escherichia coli M15(pREP4) and detected with monoclonal antibodies in the outer membrane fraction. Comp...
متن کاملHemin-dependent growth stimulation and cytochrome synthesis in Corynebacterium pyogenes.
Growth of Corynebacterium pyogenes, an important pathogen in animals, was greatly increased on addition of hemin to a medium of tryptose plus mineral. The synthesis of a type b cytochrome in this organism appeared to depend on the presence of hemin in the growth medium.
متن کاملBinding and accumulation of hemin in Porphyromonas gingivalis are induced by hemin.
Although hemin is an essential nutrient for the black-pigmented oral bacterium Porphyromonas gingivalis, the mechanisms involved in hemin binding and uptake are poorly defined. In this study, we have examined the binding of hemin and Congo red (CR) to P. gingivalis whole cells and have defined the conditions for maximal binding. Additionally, the accumulation of hemin by P. gingivalis under gro...
متن کاملHemin-binding proteins as potent markers for serological diagnosis of infections with Bartonella quintana.
It is difficult to distinguish infections with different Bartonella species using commercially available immunofluorescence (indirect immunofluorescent antibody [IFA]) assay kits. To identify appropriate proteins for serodiagnosis of Bartonella quintana infections, we investigated the antigenicity of B. quintana proteins using sera from homeless people with high B. quintana IgG titers in IFA as...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2000
ISSN: 0378-1097
DOI: 10.1016/s0378-1097(00)00243-3